JPIAMR 14th Call Partner Search Tool

General Information

• Project title: Developing feed supplement based probiotic targets that can used as effective antibiotic replacement
• Type: Project looking for partner
• Organisation: BIOVAC Nepal Pvt. Ltd.
• Country: Nepal (NP)

Research area

• Scientific area(s) of the call:
  1. Design and implementation of innovative tools, including novel chemistry and/or new materials for improved application, efficacy and delivery of antimicrobials
• One Health Setting:

  Animal Health (including wild-life, livestock, fishes, and companion animals)

• Type of studies/experimental approaches:

  in silico, in vitro, in vivo preclinical and clinical studies in human and in all veterinary settings

• Keywords:

  probiotic anti-microbial

• Brief description of your expertise / expertise you are looking for:

  poultry based probiotic target developer to be used against AMR in feed supplement

• Brief description of your project / the project you would like to join:

  Excessive and improper use of antibiotics in poultry has been contributing to the emergence of antibiotics resistant bacteria. Drug resistant infections are becoming one of the most serious concerns in poultry production, with ramifications in both human and poultry health. Increasing resistance to broad class of antibiotics has created new challenges in novel antibiotics development. Various alternative solutions are being sought for this problem, including search for effective probiotics. Our study aimed to grow and isolate potential probiotic lactic acid bacteria from indigenous chicken (Gallus gallus domesticus) that were known to be free from antibiotic exposure. The probiotic isolates were tested in vitro for their inhibition effectiveness against antibiotic resistant pathogenic bacteria using microbial and molecular techniques. A total of 90 isolates were selected from 41 cloacal samples collected from chicken at various elevation (1452- 2118 meter above sea level). Preliminary probiotic identification was carried out on selective agar medium containing calcium carbonate and L-cysteine. Out of the 90 cultured isolates of lactic acid bacteria, only 8 isolates were shortlisted as potential probiotic targets based on inhibition assay against certain pathogens (Salmonella typhi, Escherichia coli, Shigella sonnei, Klebsiella (ATCC), Citrobacter freundi and Staphylococcus aureus), lactic acid production, acid and bile salt tolerance. These tests were specifically performed to determine suitability of these isolates as potential probiotic poultry feed supplement. Majority (70%) of these selected isolates showed tolerance towards high acidic condition (pH 3.0); and more than 64.5% of the isolates survived various bile salt concentration (0%, 0.3%, 0.5% and 1%). Molecular analysis of 16S rRNA sequencing revealed one of the isolates to be Lactobacillus reuteri ATCC and remaining three were identified to be Lactobacillus plantarum PON100418. Our study has identified eight potential probiotic targets that could be used as poultry feed supplement instead of antibiotics to reduce and mitigate AMR infections in poultry production. We are looking to further this research and develop probiotic based feed supplement that is effective against AMR and replaces antibiotics use in poultry.

Dr Dibesh Karmacharya

• Organisation: BIOVAC Nepal Pvt. Ltd.
• Position: CEO/CTO
• E-mail: dibesh@cmdn.org
• Phone: +9779851110329
• Web: www.biovacnepal.com